High-Resolution Imaging of Intracellular Calcium Fluctuations Caused by Oscillating Microbubbles

Journal article (2020)

Authors

Inés Beekers Erasmus Medical Centre
D.I. Beekers Erasmus Medical Centre
Ines Beekers Erasmus Medical Centre
Frits Mastik Erasmus Medical Centre
F. Mastik Erasmus Medical Centre
Robert Beurskens Erasmus Medical Centre
Robert Beurskens Erasmus Medical Centre
R. H.S.H. Beurskens Erasmus Medical Centre
Robert H.S.H. Beurskens Erasmus Medical Centre
Phoei Ying Tang Erasmus Medical Centre
Merel Vegter Erasmus Medical Centre
Antonius F. W. van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
AFW Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F W van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F W van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
AFW Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F.W. van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F. W. Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Ton Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A.F.W. Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F. W. Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F.W. Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
AFW van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F.W. van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F W Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F W Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F W van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A.F.W. van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Ton van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F.W. Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F W Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A.F.W. Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Ton van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Afw van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F. W. van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Ton Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F W van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F.W. van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A.F.W. van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Afw Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Afw Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F.W. Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Afw van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F.W. Van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
A. F.W. van der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
AFW van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius F W Van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Antonius van Der Steen Erasmus Medical Centre, ImPhys/Medical Imaging - AS
N de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. W. Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. W. de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nico De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nico De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nico Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nicolaas De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. W. De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. de De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nico De de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nicolaas Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nicolaas de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nico De De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. De Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
Nico de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
N. de de Jong ImPhys/Medical Imaging - AS , Erasmus Medical Centre
MD Verweij Erasmus Medical Centre, ImPhys/Medical Imaging - AS
M.D. Verweij Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Martin D. Verweij Erasmus Medical Centre, ImPhys/Medical Imaging - AS
M. Verweij Erasmus Medical Centre, ImPhys/Medical Imaging - AS
M. D. Verweij Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Martin Verweij Erasmus Medical Centre, ImPhys/Medical Imaging - AS
Klazina Kooiman Erasmus Medical Centre
Research Group
ImPhys/Medical Imaging (AS) (TU Delft)
Copyright: © 2020 D.I. Beekers, Frits Mastik, Robert Beurskens, Phoei Ying Tang, Merel Vegter, A.F.W. van der Steen, N. de Jong, M.D. Verweij, Klazina Kooiman
DOI: https://doi.org/10.1016/j.ultrasmedbio.2020.03.029
More Info
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Published Date

2020

Language

English

Faculty

Applied Sciences

Department

ImPhys/Imaging Physics

Research Group

ImPhys/Medical Imaging

Abstract

Ultrasound insonification of microbubbles can locally enhance drug delivery, but the microbubble–cell interaction remains poorly understood. Because intracellular calcium (Cai 2+) is a key cellular regulator, unraveling the Cai 2+ fluctuations caused by an oscillating microbubble provides crucial insight into the underlying bio-effects. Therefore, we developed an optical imaging system at nanometer and nanosecond resolution that can resolve Cai 2+ fluctuations and microbubble oscillations. Using this system, we clearly distinguished three Cai 2+ uptake profiles upon sonoporation of endothelial cells, which strongly correlated with the microbubble oscillation amplitude, severity of sonoporation and opening of cell–cell contacts. We found a narrow operating range for viable drug delivery without lethal cell damage. Moreover, adjacent cells were affected by a calcium wave propagating at 15 μm/s. With the unique optical system, we unraveled the microbubble oscillation behavior required for drug delivery and Cai 2+ fluctuations, providing new insight into the microbubble–cell interaction to aid clinical translation.