The modulus of asphalt mixture is traditionally measured from the laboratory dynamic modulus test. However, different laboratory test methods often lead to obviously different test results. To evaluate the moduli of asphalt mixtures as constructed in field pavements, this study back-calculated the moduli of the pavement layers, based on measured strain data in Accelerated Pavement Testing (APT). Field tests were conducted to measure strains at different locations of the built pavement section at different temperatures and wheel motion speed. The loading frequencies of the asphalt layer subject to different motion speeds were calculated based on the duration of measured strain pulses. Subsequently, the relationship between the motion speed and the loading frequency was established. An finite element (FE) model of the pavement section was created, which was used to back-calculate the moduli of asphalt pavement layers using the measured strain data. Based on the back-calculation results at different loading conditions, the master curve of the field asphalt layer was determined. This master curve was further used to compare with that obtained from laboratory uniaxial compressive test. The relationship between the field and laboratory moduli was determined to be E_Laboratory= 1.298E_Field.