Fighting Antibiotic-Resistant Bacterial Infections by Surface Biofunctionalization of 3D-Printed Porous Titanium Implants with Reduced Graphene Oxide and Silver Nanoparticles

Journal article (2022)

Authors

H. San Henan Polytechnic University, Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
A.A. Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Amir A. Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Amir Abbas Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
A. A. Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
M.B. Minneboo Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
M. Minneboo Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
I. A.J. van Hengel Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Ingmar A.J. van Hengel Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
I.A.J. van Hengel Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
A. Yilmaz Team Yaiza Gonzalez Garcia - Mechanical, Maritime and Materials Engineering
Y. Gonzalez Garcia Team Yaiza Gonzalez Garcia - Mechanical, Maritime and Materials Engineering
Ad C. Fluit University Medical Center Utrecht
A. C. Fluit University Medical Center Utrecht
P.L. Hagedoorn BT/Biocatalysis - AS
Lidy E. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
E.L. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
L. E. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Lidy Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Julian Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
I. Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Research Group
Biomaterials & Tissue Biomechanics (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2022 H. San, M. Paresoglou, M.B. Minneboo, I.A.J. van Hengel, A. Yilmaz, Y. Gonzalez Garcia, Ad C. Fluit, P.L. Hagedoorn, E.L. Fratila-Apachitei, I. Apachitei, A.A. Zadpoor
DOI: https://doi.org/10.3390/ijms23169204
More Info
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Published Date

2022

Language

English

Faculty

Mechanical, Maritime and Materials Engineering

Department

Biomechanical Engineering

Research Group

Biomaterials & Tissue Biomechanics

Abstract

Nanoparticles (NPs) have high multifunctional potential to simultaneously enhance implant osseointegration and prevent infections caused by antibiotic-resistant bacteria. Here, we present the first report on using plasma electrolytic oxidation (PEO) to incorporate different combinations of reduced graphene oxide (rGO) and silver (Ag) NPs on additively manufactured geometrically ordered volume-porous titanium implants. The rGO nanosheets were mainly embedded parallel with the PEO surfaces. However, the formation of ‘nano-knife’ structures (particles embedded perpendicularly to the implant surfaces) was also found around the pores of the PEO layers. Enhanced in vitro antibacterial activity against methicillin-resistant Staphylococcus aureus was observed for the rGO+Ag-containing surfaces compared to the PEO surfaces prepared only with AgNPs. This was caused by a significant improvement in the generation of reactive oxygen species, higher levels of Ag+ release, and the presence of rGO ‘nano-knife’ structures. In addition, the implants developed in this study stimulated the metabolic activity and osteogenic differentiation of MC3T3-E1 preosteoblast cells compared to the PEO surfaces without nanoparticles. Therefore, the PEO titanium surfaces incorporating controlled levels of rGO+Ag nanoparticles have high clinical potential as multifunctional surfaces for 3D-printed orthopaedic implants.