Green roofs have been widely recognized as sustainable nature-based solutions to mitigate floods in urban areas, which, in the last decades, are increasing due to the combination of intense worldwide urbanization and climate change. Besides flood mitigation, green roofs provide additional benefits for the urban environment (e.g., reducing the urban heat island and ensuring energy saving for the underneath building). Moreover, green roofs facilitate the increase of urban biodiversity, attracting different species of small animals, and upgrade the city aesthetic value. Among the different types of green roofs, multilayer blue-green roofs present an additional layer to store water during rainfall events. As part of the Polder Roof field lab project, prototypes of multilayer blue-green roof developed by the Dutch company Metropolder were installed in four Italian cities: Cagliari, Palermo, Perugia, and Viterbo. The four prototypes and the experimental set up are described and the potential benefits of this innovative solution are discussed. Preliminary analyses, from December 2020 to December 2021, enable to estimate runoff reduction and thermal properties of multilayer blue-green roofs, underlying the high potential of this nature-based solution, which allows to retain most of the rainfall events and to mitigate the daily temperature variability.