Micro/nanoscale additive manufacturing provides a powerful tool for advanced materials and structures with complex and precise features. For instance, the feature resolution of two-photon polymerization (2PP) can reach 200 nm. At this scale, materials properties can change, and the influence of the size effect cannot be ignored. Therefore, it is necessary to assess changes in the material mechanical properties considering size effects. In this work, several micrometric polymeric specimens are printed via 2PP, and their mechanical properties are assessed using compression tests. Detailed printing and testing procedures and the effects of parameter settings are provided. The experimental results show that the changes in the microstructures size have a direct effect on Young s modulus. In particular, a large surface-volume ratio results in a higher Young s modulus. In other words, the smaller the structure size, the higher the stiffness. The reported findings play a significant role in the development of fabrication strategies for polymeric microstructures where high stiffness accuracy is fundamental.