In the last ten years (2009-2019), flooding caused the death of over 48,000 people, and affected over 697 million people globally. This is expected to increase as a result of climate change, population growth and urbanisation. Floods can cause infections due to the release of water-borne pathogens from surcharged combined sewers and other sources of faecal contamination on urban surfaces such as concrete, asphalt, gravel, pavement, playground rubber tiles and grass. Using laboratory experiments with faecal indicator bacteria Escherichia coli, and with Bacillus subtilis spores, and MS2 bacteriophages under controlled exposure to simulated sunlight, this research contributes towards a better understanding of the environmental parameters that affect the concentration of pathogens in contaminated shallow water bodies and on different urban surfaces. Also, several sampling methods are assessed for the recovery of bacteria from flood-prone urban surfaces. This study suggests that given the sunlight conditions after an urban flood, the concentration of indicator organisms and of total suspended solids and the surface type it is possible to estimate the fate of selected pathogens. The observations and results presented in this study contribute to the development of policy-making tools for rapid implementation of appropriate measures to mitigate public health risks after flooding. @en

The high frequency and intensity of urban floods caused by climate change, urbanisation and infrastructure failures increase public health risks when the flood water contaminated from combined sewer overflows (CSOs) or other sources of faecal contamination remains on urban surfaces. This study contributes to a better understanding of the effects of urban and recreational surfaces on the occurrence of waterborne pathogens. The inactivation of selected indicator organisms was studied under controlled exposure to artificial sunlight for 6 h followed by 18 h in dark conditions. Concrete, asphalt, pavement blocks and glass as control were inoculated with artificial floodwater containing, as indicator organisms, Escherichia coli bacteria, which are common faecal indicator bacteria (FIB) for water quality assessment, Bacillus subtilis spores chosen as surrogates for Cryptosporidium parvum oocysts and Giardia cysts, and bacteriophages MS2 as indicators for viral contamination. On practically all the surfaces in this study, E. coli had the highest inactivation under light conditions followed by MS2 and B. subtilis, except asphalt where MS2 was inactivated faster. The highest inactivation under light conditions was seen with E. coli on a concrete surface (pH 9.6) with an inactivation rate of 1.85 h⁻¹. However, the pH of the surfaces (varying between 7.0 and 9.6) did not have any influence on inactivation rates under dark conditions. MS2 bacteriophage had the highest inactivation under light conditions on asphalt with a rate of 1.29 h⁻¹. No die-off of B. subtilis spores was observed on any of the surfaces during the experiment, neither in light nor in dark conditions. This study underpins the need to use different indicator organisms to test their inactivation after flooding. It also suggests that given the sunlight conditions, concentration of indicator organisms and type of surface, the fate of waterborne pathogens after a flood could be estimated.

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In the last decade, flooding has caused the death of over 60,000 people and affected over 900 million people globally. This is expected to increase as a result of climate change, increased populations and urbanisation. Floods can cause infections due to the release of water-borne pathogenic microorganisms from surcharged combined sewers and other sources of fecal contamination. This research contributes to a better understanding of how the occurrence of water-borne pathogens in contaminated shallow water bodies is affected by different environmental conditions. The inactivation of fecal indicator bacteria Escherichia coli was studied in an open stirred reactor, under controlled exposure to simulated sunlight, mimicking the effect of different latitudes and seasons, and different concentrations of total suspended solids (TSS) corresponding to different levels of dilution and runoff. While attachment of bacteria on the solid particles did not take place, the decay rate coefficient, k (d⁻¹), was found to depend on light intensity, I (W m⁻²), and duration of exposure to sunlight, T (h d⁻¹), in a linear way (k = k_D+ 0.03·I and k = k_D+ 0.65·T, respectively) and on the concentration of TSS (mg L⁻¹), in an inversely proportional exponential way (k = k_D+ 14.57·e^{−0.02·[TSS]}). The first-order inactivation rate coefficient in dark conditions, k_D= 0.37 d⁻¹, represents the effect of stresses other than light. This study suggests that given the sunlight conditions during an urban flood, and the concentration of indicator organisms and TSS, the above equations can give an estimate of the fate of selected pathogens, allowing rapid implementation of appropriate measures to mitigate public health risks.

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A better understanding of the effects of different urban and recreational surfaces on the die-off of water-borne pathogens that can cause infections after urban floods if released from surcharged combined sewers and other sources of fecal contamination is needed. The die-off of fecal indicator Escherichia coli was studied under controlled exposure to simulated sunlight on a range of different surfaces found in urban environments: gravel, sand, asphalt, pavement blocks, concrete, playground rubber tiles and grass, using glass as control. The surfaces were inoculated with artificial flooding water containing 10⁵ colony forming units (CFU) of E. coli per mL and sampled periodically using the sterile cotton swab technique, after lowering the water level. The results show that dark inactivation was not statistically significant for any surface, suggesting that chemical composition and pH (varying between 6.5 ± 0.8 and 9.2 ± 0.4) did not affect the die-off rates. The highest light-induced die-off rates for E. coli after the floodwater recession, observed on rubber (>3.46 h⁻¹) and asphalt (2.7 h⁻¹), were attributed to temperature stress and loss of surface moisture.

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