Acropora palmata is one of the major reef-building coral species in the Caribbean. The species has suffered drastic declines in abundance and sexual recruitment over the past decades. One method for active rehabilitation of A. palmata reefs is by assisting the production of sexual recruits under controlled lab conditions. Within this study, the effect of different aquaculture regimes and culturing periods on the survival rates of these recruits was investigated. In August 2016, coral spawn was collected on a reef nearshore New Providence, Bahamas, cross-fertilized, and reared in mobile laboratory facilities from Van Oord Dredging and Marine Contractors. Larvae were settled on pre-conditioned aragonite plugs. Sexual recruits were cultured under four different aquaculture conditions: ambient vs. high Total Alkalinity (TA) (∼2.8 mEq L⁻¹ vs. 4.8 mEq L⁻¹) and with vs. without feeding Artemia nauplii. Recruit size was monitored by tracking living tissue area and the number of polyps of a subset of recruits. Plates with recruits were outplanted to a nursery on the reef after 4, 9 and 14 weeks of aquaculture. Survival was determined during the aquaculture phase (at 4, 9 and 14 weeks after settlement), and after outplanting (at 27 and 44 weeks after settlement). During the aquaculture phase, survival was significantly lower in seawater with increased TA compared to ambient seawater conditions. The average number of polyps per recruit was significantly higher in the treatments with feeding. After outplanting to the reef, both survival and recruit size were highest in the feeding treatments. The most successful aquaculture treatment in this study was a combination of increased TA and feeding during 9 weeks of aquaculture, which resulted in a doubling of survival and recruit size at 10 months after settlement compared to ambient conditions. Ambient conditions did not enhance survivorship nor recruit size at 10 months after settlement, as compared to the other aquaculture treatments. Nevertheless, the success of ambient aquaculture conditions exceeded natural conditions, as no natural recruitment of A. palmata was observed in this study. We conclude that feeding during and ex-situ culture period enhances ex-situ growth rates and in situ recruit survival of A. palmata juveniles. No positive effects of the aquaculture treatment with only increased TA were found. Building on these results, recommendations are provided for future reef rehabilitation efforts using ex-situ rearing of sexually reproduced A. palmata recruits.

The North Sea was once abundantly covered with hard substrates such as oyster beds, coarse peat banks and glacial erratics, providing habitat to a rich community of marine species. Most of these habitats were destroyed by bottom-trawl fisheries over the past century, and today, the seabed hosts a relatively poor species community. Emerging offshore windfarms include the re-introduction of hard substrate by means of scour protection around the foundation of wind turbines. It is assumed that the new habitat will contribute to marine biodiversity, and this study aims to demonstrate that. Video data were collected using a Remotely Operated Vehicle in four wind farms in the southern North Sea. A quantitative assessment was made to determine the effect of scour protection on community structure. The assessment revealed distinct community clusters for geographic location and seabed type. Windfarms closely located to each other had a more similar epibenthic community compared to those further away. The epibenthic community at the rocky armour layer of the scour protection had a different species composition and a higher species abundance than the one at the sandy seabed surrounding it. Species diversity by means of richness, evenness and the Shannon diversity index was not consistently higher or lower for the communities at the different seabed types. This study shows that marine life inhabits scour protection in offshore wind farms and that it is different from the community living at the surrounding seabed. Knowing the potential epibenthic community structure at and around a scour protection supports the development of new wind farms that include components to enhance their ecological value. Herewith, our study contributes to efforts to restore biodiversity in the North Sea.