Accessibility is a fundamental concept concerning urban and transport planning as it is the elementary basis for socio-economic development in cities. It can be described as the potential to reach spatially distributed opportunities. Recent research has identified that excluding person-based features from this analysis might cause inaccurate measurement of accessibility. Age, gender, and income, for instance, are responsible for varying accessibility levels drastically. In addition, studies worldwide have shown that women face different challenges in reaching locations and spatially distributed opportunities. Despite the findings, this research identifies a gap in understanding how a person-based perspective, mainly gender, and other personal characteristics, affect accessibility levels, considering various travel purposes and transport modes. Thus, this research aims to answer the question, ”How do person-based characteristics, mainly gender, can impact accessibility levels?”. The primary objective is to explore how these characteristics influence accessibility metrics, identify the urban groups most affected by the absence of this perspective, and determine the key personal characteristics that significantly impact accessibility levels. To address these objectives, this study considers a combination of quantitative and case-study research approaches. It investigates the Metropolitan Region Rotterdam- The Hague in The Netherlands by applying surveys to obtain perceived accessibility data. In the same region, spatial analysis is conducted by mapping transport networks and points of interest. Then, this study compares spatially calculated accessibility with self-reported accessibility and the presence of mismatches. In addition, cluster analysis identifies the urban profiles most vulnerable to mismatches and their main characteristics. A Binary logistic regression is conducted to determine the variables’ importance in the mismatch occurrence. From the survey answers, it is identified that women have less access to cars than men. In addition, the comparison between perceived accessibility and spatial accessibility uncovers that women present the most critical mismatches to reach activities by car. In other words, several women perceive the car as an impractical option to access points of interest that are spatially considered reachable by car. It raises the hypothesis that the lack of car access highly impacts their accessibility perception. Furthermore, the clustering analysis reveals that foreign women exhibit a higher prevalence of car-related mismatches when compared to other urban groups. Moreover, this research identifies that fathers of young children also encounter greater disparities across all transportation modes. Additionally, the binary logistic regression underscores the importance of safety as a critical factor influencing women’s perception of walking as a viable mode of transportation. This safety importance is also identified from the survey answers. The findings raise new hypotheses that warrant investigation in transport engineering, urban planning, and social sciences. Consequently, this research can contribute to developing more inclusive transport policies and establishing a more equitable transport system.