Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

Journal article (2008)

Authors

Horia L. Banciu Interdisciplinary Research Institute on Bio-Nano-Science of Babes-Bolyai University

Dimitry Y. Sorokin BT/Environmental Biotechnology - , Russian Academy of Sciences

Tatiana Tourova Russian Academy of Sciences

Erwin A. Galinski Universität Bonn

Maria S. Muntyan Moscow State University

J.G. Kuenen BT/Environmental Biotechnology -

G. Muijzer BT/Environmental Biotechnology -

Research Group

BT/Environmental Biotechnology () (TU Delft)

Soda lakes Haloalkaliphilic Halothiobacillus Sulfur-oxidizing bacteria Thioalkalibacter halophilus

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Published Date

2008-5

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Environmental Biotechnology

Abstract

A chemolithoautotrophic sulfur-oxidizing bacterium (SOB) strain ALCO 1 capable of growing at both near-neutral and extremely alkaline pH was isolated from hypersaline soda lakes in S-W Siberia (Altai, Russia). Strain ALCO 1 represents a novel separate branch within the halothiobacilli in the Gammaproteobacteria, which, so far, contained only neutro-halophilic SOB. On the basis of its unique phenotypic properties and distant phylogeny, strain ALCO 1 is proposed as a new genus and species Thioalkalibacter halophilus gen. nov. sp. nov. ALCO 1 was able to grow within a broad range of salinity (0.5-3.5 M of total sodium) with an optimum at around 1 M Na+, and pH (7.2-10.2, pHopt at around 8.5). Na+ was required for sulfur-dependent respiration in ALCO 1. The neutral (NaCl)-grown chemostat culture had a much lower maximum growth rate (μmax), respiratory activity and total cytochrome c content than its alkaline-grown counterpart. The specific concentration of osmolytes (ectoine and glycine-betaine) produced at neutral pH and 3 M NaCl was roughly two times higher than at pH 10 in soda. Altogether, strain ALCO 1 represents an interesting chemolithoautotrophic model organism for comparative investigations of bacterial adaptations to high salinity and pH.