In Vivo Prevention of Implant-Associated Infections Caused by Antibiotic-Resistant Bacteria through Biofunctionalization of Additively Manufactured Porous Titanium

Journal article (2023)

Authors

I. A.J. van Hengel Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
I.A.J. van Hengel Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Ingmar A.J. van Hengel Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Bruce van Dijk University Medical Center Utrecht
A.A. Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Amir A. Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Amir Abbas Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
A. A. Zadpoor Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
K. Modaresifar Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
J. Fred F. Hooning van Duyvenbode University Medical Center Utrecht
F. Ruben H.A. Nurmohamed University Medical Center Utrecht
Sander A. Leeflang Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
M.A. Leeflang Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
A. C. Fluit University Medical Center Utrecht
L. E. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Lidy E. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Lidy Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
E.L. Fratila-Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Julian Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
I. Apachitei Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Harrie Weinans Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering , University Medical Center Utrecht
HH Weinans Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering , University Medical Center Utrecht
H. Weinans Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering , University Medical Center Utrecht
Research Group
Biomaterials & Tissue Biomechanics (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2023 I.A.J. van Hengel, B. van Dijk, K. Modaresifar, J. Fred F. Hooning van Duyvenbode, F. Ruben H.A. Nurmohamed, M.A. Leeflang, A.C. Fluit, E.L. Fratila-Apachitei, I. Apachitei, Harrie Weinans, A.A. Zadpoor
DOI: https://doi.org/10.3390/jfb14100520
More Info
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Published Date

2023

Language

English

Faculty

Mechanical, Maritime and Materials Engineering

Department

Biomechanical Engineering

Research Group

Biomaterials & Tissue Biomechanics

Abstract

Additively manufactured (AM) porous titanium implants may have an increased risk of implant-associated infection (IAI) due to their huge internal surfaces. However, the same surface, when biofunctionalized, can be used to prevent IAI. Here, we used a rat implant infection model to evaluate the biocompatibility and infection prevention performance of AM porous titanium against bioluminescent methicillin-resistant Staphylococcus aureus (MRSA). The specimens were biofunctionalized with Ag nanoparticles (NPs) using plasma electrolytic oxidation (PEO). Infection was initiated using either intramedullary injection in vivo or with in vitro inoculation of the implant prior to implantation. Nontreated (NT) implants were compared with PEO-treated implants with Ag NPs (PT-Ag), without Ag NPs (PT) and infection without an implant. After 7 days, the bacterial load and bone morphological changes were evaluated. When infection was initiated through in vivo injection, the presence of the implant did not enhance the infection, indicating that this technique may not assess the prevention but rather the treatment of IAIs. Following in vitro inoculation, the bacterial load on the implant and in the peri-implant bony tissue was reduced by over 90% for the PT-Ag implants compared to the PT and NT implants. All infected groups had enhanced osteomyelitis scores compared to the noninfected controls.