Optimal model-based sensorless adaptive optics for epifluorescence microscopy

Journal article (2018)

Authors

P. Pozzi Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
O.A. Soloviev Flexible Optical B.V., Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering , ITMO University
D. Wilding Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Gleb Vdovin Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering , Flexible Optical B.V., ITMO University
M.H.G. Verhaegen Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
Research Group
Team Raf Van de Plas (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2018 P. Pozzi, O.A. Soloviev, D. Wilding, Gleb Vdovin, M.H.G. Verhaegen
DOI: https://doi.org/10.1371/journal.pone.0194523
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Published Date

2018

Language

English

Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Raf Van de Plas

Abstract

We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image quality and the aberration. The method is potentially applicable to any form of microscopy with epifluorescence detection, including the practically important case of incoherent fluorescence emission from a three dimensional object, through minor hardware modifications. We have applied the technique successfully to a widefield epifluorescence microscope and to a multiaperture confocal microscope.