Growth factor-induced osteogenesis in a novel radiolucent bone chamber

Journal article (2015)

Authors

MT Poldervaart External organisation
J van der Stok External organisation
MFP de Haas External organisation
MC 't Hart External organisation
FC Öner External organisation
Wouter J.A. Dhert External organisation
H. Weinans University Medical Center Utrecht, Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Harrie Weinans University Medical Center Utrecht, Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
HH Weinans University Medical Center Utrecht, Biomaterials & Tissue Biomechanics - Mechanical, Maritime and Materials Engineering
Jacqueline Alblas External organisation
Research Group
Biomaterials & Tissue Biomechanics (Mechanical, Maritime and Materials Engineering) (TU Delft)
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Published Date

2015

Language

English

Reuse Rights

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Biomechanical Engineering

Research Group

Biomaterials & Tissue Biomechanics

Abstract

Treatment of large bone defects is currently performed using mainly autograft or allograft bone. There are important drawbacks to bone grafting, such as limited availability, donor site morbidity in the case of autograft and inferior performance of allografts. Therefore, there is a great need for a suitable bone graft substitute. In order to evaluate efficiently newly developed biomaterials and factors intended for orthopaedic surgery, the bone chamber is a very suitable model. To allow longitudinal investigation of bone growth with μCT, a new bone chamber made of radiolucent polyether ether ketone (PEEK) was developed and studied for its feasibility. Therefore, PEEK bone chambers were placed on rat tibiae, and filled with vehicle (Matrigel without growth factors, negative controls), with bone morphogenetic protein 2 (BMP-2, positive controls), or a mix of growth factors combining BMP-2, vascular endothelial growth factor and the chemokine stromal cell-derived factor 1α, all laden on gelatin microspheres for controlled release (combined growth factors). Growth factor presence led to a significant increase in bone formation after 8 weeks, which subsided after 12 weeks, underlining the importance of longitudinal analysis. We conclude that the PEEK-bone chamber is a suitable translational animal model to assess orthotopic bone formation in a longitudinal manner.