Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

Journal article (2017)

Authors

Xu Wang South China University of Technology
Peng Fei Zhou South China University of Technology
Gang Li South China University of Technology
Bo Yang South China University of Technology
Frank Hollmann BT/Biocatalysis - AS
F. Hollmann BT/Biocatalysis - AS
Yong Hua Wang South China University of Technology
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2017 Xu Ping Wang, Peng Fei Zhou, Zhi Gang Li, Bo Yang, F. Hollmann, Yong Hua Wang
DOI: https://doi.org/10.1038/srep44599
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Published Date

20-03-2017

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.