Single-cell immune profiling reveals thymus-seeding populations, T cell commitment, and multilineage development in the human thymus

Journal article (2022)

Authors

Martijn Cordes Leiden University Medical Center
Kirsten Canté-Barrett Leiden University Medical Center
M. J.T. Reinders Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
Marcel Reinders Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
Marcel J.T. Reinders Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
M.J.T. Reinders Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
F.J.T. Staal Leiden University Medical Center
Erik Ben van den Akker Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
E. B. van den Akker Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
Erik van den Akker Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
Erik B. van den Akker Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
E.B. van den Akker Pattern Recognition and Bioinformatics - EEMCS , Leiden University Medical Center
Federico A. Moretti Leiden University Medical Center
Szymon M. Kiełbasa Leiden University Medical Center
Sandra A. Vloemans Leiden University Medical Center
Laura Garcia-Perez Leiden University Medical Center
Cristina Teodosio Leiden University Medical Center, University of Salamanca
Jacques J.M. van Dongen University of Salamanca, Leiden University Medical Center
K. Pike-Overzet Leiden University Medical Center
Research Group
Pattern Recognition and Bioinformatics (EEMCS) (TU Delft)
Copyright: © 2022 Martijn Cordes, Kirsten Canté-Barrett, E.B. van den Akker, Federico A. Moretti, Szymon M. Kiełbasa, Sandra A. Vloemans, Laura Garcia-Perez, Cristina Teodosio, Jacques J.M. van Dongen, K. Pike-Overzet, M.J.T. Reinders, F.J.T. Staal
DOI: https://doi.org/10.1126/sciimmunol.ade0182
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Published Date

2022

Language

English

Reuse Rights

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Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Intelligent Systems

Research Group

Pattern Recognition and Bioinformatics

Abstract

T cell development in the mouse thymus has been studied extensively, but less is known regarding T cell development in the human thymus. We used a combination of single-cell techniques and functional assays to perform deep immune profiling of human T cell development, focusing on the initial stages of prelineage commitment. We identified three thymus-seeding progenitor populations that also have counterparts in the bone marrow. In addition, we found that the human thymus physiologically supports the development of monocytes, dendritic cells, and NK cells, as well as limited development of B cells. These results are an important step toward monitoring and guiding regenerative therapies in patients after hematopoietic stem cell transplantation.

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